Summary of GFP-HA tagged library construction |
The schematic diagrams of library construction |
Summary of experimental procedures |
| Experiental Category | No. of ORF | Efficiency [%] |
|---|---|---|
| Total ORF | 1.630 | |
| First PCR | 1.580 | 97 |
| Second PCR | 1.537 | 94 |
| Transformation | 1.468 | 90 |
| Confirmed integration | 1.058 | 65 |