Depletion of autophagy receptor p62/SQSTM 1 enhances the efficiency of gene delivery in mammalian cells
M Tsuchiya, H Ogawa, T Koujin, S Kobayashi… - FEBS …, 2016 - Wiley Online Library
FEBS letters, 2016•Wiley Online Library
Novel methods that increase the efficiency of gene delivery to cells will have many useful
applications. Here, we report a simple approach involving depletion of p62/SQSTM 1 to
enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was
remarkably higher in p62‐knockout murine embryonic fibroblast (MEF) cells compared with
normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of
p62. Furthermore, si RNA‐mediated knockdown of p62 clearly increased the efficiency of …
applications. Here, we report a simple approach involving depletion of p62/SQSTM 1 to
enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was
remarkably higher in p62‐knockout murine embryonic fibroblast (MEF) cells compared with
normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of
p62. Furthermore, si RNA‐mediated knockdown of p62 clearly increased the efficiency of …
Novel methods that increase the efficiency of gene delivery to cells will have many useful applications. Here, we report a simple approach involving depletion of p62/SQSTM1 to enhance the efficiency of gene delivery. The efficiency of reporter gene delivery was remarkably higher in p62‐knockout murine embryonic fibroblast (MEF) cells compared with normal MEF cells. This higher efficiency was partially attenuated by ectopic expression of p62. Furthermore, siRNA‐mediated knockdown of p62 clearly increased the efficiency of transfection of murine embryonic stem (mES) cells and human HeLa cells. These data indicate that p62 acts as a key regulator of gene delivery.
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