ラボ マニュアル
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1. Supporting Materials and Methods
- 1.01 Lab Water
- 1.02 DNA Sequencing
- 1.03 Site-Directed Mutagenesis
- 1.04 Southern Blotting
- 1.05 Pulse-Field Gel Electrophoresis
- 1.06 Northern Blotting
- 1.07 In vitro Protein Expression
- 1.08 Quantitation of Protein
- 1.09 SDS-PAGE
- 1.10 Immunoblotting
- 1.11 Flow Cytometry
- 1.12 Two-Hybrid Screening
2. Working with E.coli
- 2.01 Cuture Medium
- 2.02 Competent Cells
- 2.03 Transformation
- 2.04 Colony PCR
- 2.05 Protein Expression and Purification
3. Working with S. pombe
- 3.01 Cuture Medium
- 3.02 Culture
- 3.03 Transformation
- 3.04 Colony PCR
- 3.05 Gene Disruption and tagging
- 3.06 lacO/LacI-GFP Chromosome Labeling
- 3.07 Isolation of Nuclei
- 3.08 Genomic DNA Preparation
- 3.09 RNA Preparation
- 3.10 Protein Expression and Purification
- 3.11 Immuno-precipitation (IP)
- 3.12 Inducing Meiosis
- 3.13 Synchronization of vegetative growth cell
- 3.14 Live Cell Observation
- 3.15 Immunofluorescence and FISH
- 3.16 Chromatin Immunoprecipitation (ChIP)
- 3.17 SPB Activation
- 3.18 Protoplast Fusion
4. Working with Mammalian Cells
- 4.01 Cell Culture and Storage
- 4.02 Immunofluorescence
- 4.03 Transfection
- 4.04 Microinjection
- 4.05 Live Observation
5. Working with Tetrahymena
- 5.01 Cell Culture and Storage
- 5.02 Immunofluorescence
- 5.03 Transfection
- 5.04 Microinjection
- 5.05 Live Observation
6. Working with Sf9
7. Working with DT40
- 7.01 Culture
- 7.02 Transfection
- 7.03 Subcloning
- 7.04 Conditional Mutants
- 7.05 Immunofluorescence
- 7.06 Metaphase Spreading
- 7.07 BrdU Incorporation